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CITE
U. Jain, P. Singh, J.K. Yadav, J.P. Thakur and R.P. Mishra .(2018). "Antibiogram and PCR Detection of Verotoxic Escherichia coli Recovered from Raw Milk and Burfi of Uttar Pradesh". Journal of Veterinary Public Health, Vol. 16 Issue 1. Page No: 35-38
Antibiogram and PCR Detection of Verotoxic Escherichia coli Recovered from Raw Milk and Burfi of Uttar Pradesh
Page No. : 35-38
ABSTRACT
This study was undertaken to detect Verotoxic Escherichia coli in milk and burfi using polymerase chain reaction (PCR) and screen the isolates for antibiotic susceptibility. A total of 80 raw milk and 160 burfi samples were collected and analysed for verocytotoxic E. coli (VTEC); of which 6.25 % (5/80) milk and 10.62% (17/160) burfi samples were found positive for VTEC by PCR. Antibiogram of VTEC isolates against 15 antimicrobials showed sensitivity to chloramphenicol and imipenem-EDTA (100%); followed by ciprofloxacin (90.90%), streptomycin (68.18%), amikacin and gentamicin (54.54%). While resistance was recorded against erythromycin and cefixime (100%), followed by ampicillin (45.45%), tetracycline (40.90%), enrofloxacin (31.81%) and ofloxacin (27.27%).Key words: Antibiogram, burfi, milk, PCR, VTEC