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CITE
S.S.S. Goud, A. Jagadeesh Babu and M. Shylaja.(2018). "A Study on the Incidence of Shiga Toxin Producing Escherichia Coli Isolated from Dairy Farm Sewage". Journal of Veterinary Public Health, Vol. 16 Issue 1. Page No: 45-48
A Study on the Incidence of Shiga Toxin Producing Escherichia Coli Isolated from Dairy Farm Sewage
Page No. : 45-48
ABSTRACT
The present study was undertaken for isolation and molecular characterization of Escherichia coli from dairy farm sewage and waste water samples, by using the primers derived from stx1, stx2, eaeA and hlyA genes. A total of 128 samples were collected from organized, unorganized dairy farms and also from animal sheds of small farms and isolated E. coli. All the 128 isolates were confi rmed as E. coli by subjecting them to various biochemical tests. The PCR assay revealed that of out of 28 samples from organized dairy farms one (3.57%) was positive for stx1 gene. Out of 32 samples from unorganized dairy farms, two (6.25%) samples were positive for STEC, of which one (3.13%) carried stx1 gene and another (3.13%) carried stx1, stx2 and eaeA genes. Out of 68 samples from the animal sheds, eight (11.76%) samples were positive for STEC by PCR method. Among them three (4.41%) samples carried stx1, four (5.88%) samples carried stx2 and another one (1.47%) sample carried both stx1and stx2 genes.Key words: Dairy farm sewage, Escherichia coli, PCR, STEC