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Page No. : 79-83

ABSTRACT

Brucellosis is one of the most important re-emerging zoonoses prevalent all over the world with serious public health and economic implications. The objective of this paper was to perform pulsed-field gel electrophoresis (PFGE) on field isolates of Brucella using one of the rare-cutting restriction enzymes XbaI to determine strain variations, if any. PFGE produced 7 distinct banding patterns in 73 Brucella isolates consisting of 27–28 bands ranging from approx. 10 to 600 kb. The PFGE analysis using XbaI revealed differences in the genomic fingerprints among different species with unique electrophoretic differences among B. abortus and B. melitensis field isolates as well as among different reference strains. This appears to be the first report of PFGE analysis with Indian isolates of Brucella.

Keywords: B. abortus, B. melitensis, PFGE, Zoonoses