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Page No. : 85-89

ABSTRACT

Food animals are increasingly recognized as reservoirs for ESBL-producing strains. The present study was undertaken to evaluate the status of ESBL producing Enterobacteriaceae in foods of animal origin. A total of 100food samples comprising of raw milk (40), milk products (30), raw meat (15) and meat products (15) were collected from local shops of Kumarganj and Lucknow (UP). A total of 140 enterobacterial isolates were recovered with Citrobacter (38.41%) being the most dominant flora. All the presumptive ESBL producers were tested by phenotypic and molecular methods. Phenotypic test revealed highest prevalence of ESBL producers in rawmilk (24.44%), followed by raw meat (16.0%), milk products (8.0%) and meat products (15.0%). Phenotypically positive isolates were further tested by polymerase chain reaction (PCR) for detection of ESBL genes viz. blaCTX, blaSHV and blaTEM. Highest number of ESBL producers were recorded in raw milk (9), followed byraw meat (2); however, none of the ESBL producer was recovered from milk and meat products. PCR method recorded less number of ESBL producers (11) than phenotypic method (22). The gene blaCTX was found to be most dominant (9) followed by blaSHV (4) and blaTEM (1). All enterobacterial isolates tested positive for ESBL genes belonged to the genera Escherichia (7) and Citrobacter (4).

Keywords: Antibiotic resistance, ESBL, enterobacteria, milk, meat