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Page No. : 7-12

ABSTRACT

A loop mediated isothermal amplification test was standardized for the rapid detection of Salmonella in the chicken meat samples. The LAMP primers in the study were used for targeting the invA gene of the genus Salmonella. The optimum condition for reaction was found to be 64˚C for 70 min. The detection limits of standardized invA based LAMP assay was found to be 200 fg per reaction mixture while, invA PCR detected 20 pg, which indicated that LAMP assay was 100 times more sensitive than PCR in detecting the Salmonella. The specificity of LAMP assay was estimated with the help of 10 Salmonella isolates and 6 non Salmonella isolates. The LAMP assay detected correctly all 10 Salmonella isolates tested but did not detect any of the non- Salmonella isolates, similar results were also given by the invA PCR. Two hundred twenty field chicken meat samples were screened by invA LAMP and invA PCR, of which, 17 (7.7%) samples were positive by LAMP test and 15(6.8%) samples were by PCR. LAMP assay had advantage in its simplicity, rapidity, and accuracy over PCR in detecting Salmonella from naturally contaminated chicken meat.

Keywords: Chicken meat, LAMP, rapid det