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CITE
R.V. Singh, B. Bist, K. Nagrajan, S. Das, R.S. Tayde and A.Verma .(2014). " Genotyping of Clostridium perfringens isolates from chicken by multiplex PCR". Journal of Veterinary Public Health, Vol. 12 Issue 1. Page No: 41-43
Genotyping of Clostridium perfringens isolates from chicken by multiplex PCR
Page No. : 41-43
ABSTRACT
The aim of the study was to genotype the isolates of Cl. perfringens from chicken meat with multiplex PCR. The study was targeted for 6 genes (cp, cpa, cpb, iA, etx and cpe). Genotyping of 61 isolates of Cl. perfringens (rawmeat-60 and fried meat-1) revealed that 55 (90.16%) strains had cpa genes while presence of only one isolate (1.6%) was positive for enterotoxin gene (cpe). None of the isolates showed the cpb, iA and etx genes. All the isolates were positive for cp (Cl. perfringens) gene.Keywords: Chicken, clostridium perfringens, genotyping, PCR