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CITE
P. Kaushik, Anjay and S. Dayal
.(2014). "Rapid PCR Detection of Salmonella from Stool Samples". Journal of Veterinary Public Health, Vol. 12 Issue 2. Page No: 71-74
Rapid PCR Detection of Salmonella from Stool Samples
Page No. : 71-74
ABSTRACT
Salmonellosis is one of the leading causes of acute enteric infection in humans and animals. The present study was undertaken to develop a Polymerase Chain Reaction (PCR) protocol for rapid detection of Salmonella directly from the stool samples. A PCR was optimized targeting invA gene of Salmonella. The sensitivity of the PCR was found to be 8X102 cfu/ml from serially diluted bacterial culture, whereas the sensitivity in spiked stool sample after 4 h enrichment was found up to 2X102 cfu/ml. The study showed that PCR is highly sensitive and specific diagnostic tool for rapid and specific detection of Salmonella from stool samples. This can be used for epidemiological investigations, where immediate diagnosis is needed for patient management.Keywords: invA, PCR, Salmonella, stool