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Page No. : 7-10

ABSTRACT

In the present study, 83 buffalo, 54 poultry faecal and 81 human stool samples were screened by RNA-PAGE for the presence of rotavirus, of which, 6 bovine (12.5%), 4 poultry (7.84%) and16 human (20.25%) samples were detected positive. RNA migration pattern of 4:2:3:2, characteristic of group A rotavirus was observed in bovine and human stool samples, whereas all avian faecal samples showed 5:2:2:2 migration pattern of group D avian rotavirus. Out of 26 rotavirus positive samples from different species, 6 (23.07%) bovine samples showed long electropherotype, while in avian, all the 4 (15.38%) samples revealed short migration pattern and out of 16 human samples, 9 (56.25%) showed long RNA migration pattern and 6 (37.5%) had short electropherotype, while 1 (6.25%) was of non group A rotavirus. In this study a total of 10 different electropherotypes were identified among the different host species at different locations indicating wide spread genomic diversity among rotaviruses prevalent in the region. The study also reports first ever detection of group D avian rotavirus in Maharashtra.

Keywords: Genomic diversity, RNA- PAGE, rotavirus