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CITE
D.D. Katre, N.N. Zade, S.V. Shinde, A.D. Jaulkar, W.A. Khan and S.P. Chaudhari.(2010). "R – Plasmid Curing of Listeria species from Foods of Animal Origin". Journal of Veterinary Public Health, Vol. 08 Issue 1. Page No: 57-59
R – Plasmid Curing of Listeria species from Foods of Animal Origin
Page No. : 57-59
ABSTRACT
Of the nine isolates of Listeria species tested for plasmid isolation and their characterization, eight harbored one to three plasmid fragments of varying molecular size between 16148.78 bp to698.00 bp. All plasmid bearing Listeria isolates resistant to azithromycine were subjected to the presence of plasmid mediated drug resistance using glycine as a chemical agent. The curing experiment revealed elimination of plasmid of molecular size 9117.78 bp from only one isolate of Listeria murrayi. The frequency of curing was found to be 100 per cent. The remaining isolates of Listeria spp. failed to grow after addition of glycine in the BHI broth.Keywords: Curing, Listeria species, Plasmid