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CITE
Prejit, R.K. Agarwal, K. Porteen, K. Kumar, Shweta Singh, Sangeeta Singh and Asha K..(2010). "Cloning and sequencing of outer membrane protein C (ompC) gene from Salmonella Typhimurium". Journal of Veterinary Public Health, Vol. 08 Issue 2. Page No: 111-115
Cloning and sequencing of outer membrane protein C (ompC) gene from Salmonella Typhimurium
Page No. : 111-115
ABSTRACT
Salmonella Typhimurium is among the most common bacteria responsible for foodborne gastroenteritis. Outer membrane protein C (ompC) gene, identified in the Salmonella Typhimurium genome, was cloned and custom sequenced. Predicted amino acid sequences showed a protein of 378 amino acids and presence of a large number of antigenic regions, using the Jameson-Wolf method. Hence, it may act as good immunogen and can be tried as vaccine candidate. Homology search showed nearly complete homology (99-97%) with other Salmonella OmpC protein and with varying degree of homology with OMP genes of other bacteria.Keywords: Cloning, ompC, Salmonella, vaccine candidate