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ABSTRACT

Pandemic group of Vibrio parahaemolyticus possesses a variety of ‘unique’ DNA markers, like toxRS/new sequence (GS-PCR), orf-8, a 930 bp AP-PCR fragment (PGS-PCR) and various other markers. In the present study, initially 94 tdh+ and trh- (virulent), then 75 toxR+ (species specific), V. parahaemolyticus isolates from saline water fishes of Kolkata Metropolis based fish markets were analyzed for the presence of three pandemic markers viz., toxRS/new region (GSPCR), orf-8 and PGS-PCR. Out of 94 tdh+ isolates, 58 (61.7%) were positive for toxRS/new region in GS-PCR. None of the isolates were positive for the orf-8 gene; however, in PGS-PCR assay all tested isolates (tdh+) were positive. But when 75 toxR+ non-virulent isolates were tested by all these three markers, no isolate was found positive for GS-PCR and orf-8 PCR; however, all were found positive in PGS-PCR. Thus, from the present study it may be inferred that only GS-PCR is a suitable marker for identification of pandemic group; while, orf-8 and PGS-PCR assay might not be appropriate.

Keywords: GS-PCR, orf-8, PGS-PCR, pandemic group, Vibrio parahaemolyticus