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Page No. : 99-101

ABSTRACT

An attempt was made to study the penetration pattern of Salmonella Typhimurium in desi and white shelled eggs by artificial inoculation at ambient temperature (30±20 C). A total of 120 shelled eggs comprising of 60 white shelled eggs and 60 desi eggs were distributed into 5 lots containing 12 eggs each. Out of these, 10 eggs were kept for surface inoculation with S. Typhimurium and 2 eggs as control and analyzed at 2, 24, 48, 72 and 96 h after artificial inoculation. The shell surface was decontaminated with 65 ppm of polyhexamethylene guanidine (PHMG) before analysis. The penetration of S. Typhimuriumin desi eggs was found to be within 2 h with an average count of 2.17±0.79 log cfu/ml, whereas in white shelled eggs, the penetration was after 24 h with an average bacterial count of 0.71±0.63 log cfu/ml. The penetration percentage and S. Typhimurium count inside both shelled eggs further increased from 24 h to 96 h. After 96 h, all eggs had 100% penetration with an average count of 4.72±0.01 and 4.56±0.05 log cfu/ml for desi and white shelled eggs, respectively. It can be concluded that both desi and white shelled eggs allowed penetration of S. Typhimurium with slight variation in percentage and duration for penetration at ambient temperature.

Keywords: Artificial inoculation, desi eggs, eggshell, penetration, Salmonella Typhimurium, white shelled Eggs