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Page No. : 75-79

ABSTRACT

In the present study, a total of sixteen Proteus mirabilis isolates were recovered from 80 samples comprising of chicken (8 isolates from 25 samples), pork (2 isolate from 10 samples) and chicken cloacal swabs (6 isolates from 45 samples). The isolates were confi rmed by standard cultural and biochemical tests. The molecular identifi cation of Proteus mirabilis was carried outby amplifi cation of species specifi c UreR gene (225 bp) by PCR. ERIC-PCR and rep-PCR were performed in order to assess genetic relatedness of Proteus mirabilis isolates of diff erent sources. The study indicated that, occurrence of Proteus mirabilis in pork, chicken meat and chicken cloacal swabs was 20%, 32% and 13.3%, respectively. Rep-PCR was able to discriminate the P. mirabilis isolates be" er than ERIC-PCR. Therefore, Proteus mirabilis in meat could be a potential concern in food safety.
Keywords: Chicken, cloacal swabs, ERIC-PCR, PCR, pork, Proteus mirabilis, REP-PCR