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CITE
D.K. Sinha, D.K. Singh and T.K. Goswami.(2013). "Immunoreactivity of Brucella melitensis 16M soluble antigen (BmSA) after ion-exchange chromatography". Journal of Veterinary Public Health, Vol. 11 Issue 1. Page No: 65-68
Immunoreactivity of Brucella melitensis 16M soluble antigen (BmSA) after ion-exchange chromatography
Page No. : 65-68
ABSTRACT
In the present study, soluble antigen of Brucella melitensis 16 M (BmSA) was prepared and purified by ion-exchange chromatography (DEAE-Sepharose) with linear gradient of NaCl (5 mM to 1 M) in Tris-HCl buffer (10 mM, pH 7.5). The fractions obtained constituted three distinct peaks, containing varying amount of protein and carbohydrate. The immune reactivity of these fractions in relation to lipopolysaccharide (LPS) – protein association by enzyme-linked immunosorbent assay (ELISA) is described.Keywords: Brucella melitensis, ELISA, ion-exchange chromatography, lipo-polysaccharide (LPS), soluble antigen (BmSA)