Archives

Page No. : 105-109

ABSTRACT

Arcobacters are considered potential zoonotic emerging food and waterborne pathogens. In the present study, a total of 205 samples of diverse origin comprising chicken meat (104), pork (51) and human stool (50) samples were collected from Bareilly region. A total of 65 samples were found positive for Arcobacter species by genus specific PCR method targeting 16S rRNA, giving an overall prevalence of31.7%, wherein chicken meat samples showed 39.42%, pork 37.25% and human stool samples 10%prevalence. By conventional cultural method prevalence was found to be 23.41%, where pork samples showed higher prevalence (33.33%), followed by chicken meat samples (28.85%), and human stool samples (2%). Application of multiplex PCR for Arcobacter isolates targeting 16S and 23S rRNA genes for species identification revealed prevalence of A. butzleri (39.58%), A. skirrowii (2.08%), A. cryaerophilus(25%), both A. butzleri and A. cryaerophilus (14.58%), both A. butzleri and A. skirrowii (6.25 %) and both A. skirrowii and A. cryaerophilus (6.25%) and all the three species (6.25%). High prevalence rate of Arcobacter spp., in food samples of animal origin has important public health implications.

Keywords: Arcobacter, chicken meat, isolation, pork, prevalence