-
Mail Address
vetviju@gmail.com
close
CITE
A. Kamboj, A.A. Raut, D. Senthilkumar, P. Ranaware, K. Rajukumar, A. Mishra and D.D. Kulkarni .(2013). "Amplification and cloning of full length ‘S’ segment of crimean-congo hemorrhagic fever virus from Hyalomma ticks infesting cattle in Gujarat, India". Journal of Veterinary Public Health, Vol. 11 Issue 2. Page No: 141-143
Amplification and cloning of full length ‘S’ segment of crimean-congo hemorrhagic fever virus from Hyalomma ticks infesting cattle in Gujarat, India
Page No. : 141-143
ABSTRACT
Crimean-Congo hemorrhagic fever (CCHF) is an emerging zoonotic disease in India, first reported from Gujarat, in December 2010. In the present study, CCHFV genome was detected in Hyalomma ticks infesting cattle collected in early 2011, from Undani village, of Mehsana district of Gujarat, India under post human CCHF outbreak surveillance activity. The full-length ‘S’ segment of CCHFV was amplified using one-step RT-PCR, from total RNA isolated from these ticks. The 1673 bp amplicon was further cloned in to pGEM®-T Easy vector and confirmed by restriction digestion. This full length ‘S’ segment was sequenced and sequence was submitted to NCBI GenBank database under Accession No JX051650.Keywords: Crimean-Congo hemorrhagic fever, nucleoprotein gene, ‘S’ Segment, tick-born zoonosis.