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Page No. : 149-151

ABSTRACT

The present study was carried out for plasmid profile analysis of Salmonella spp. from man, animals and foods of animal origin. A total of 50 Salmonella isolates from 1,132 different sources viz., poultry meat (212), poultry eggs (49), poultry droppings (60), autopsied poultry tissues (60), pork (156), pigfaeces (189), cattle dung (105), buffalo dung (103), sheep faeces (11), goat faeces (31), deer faeces (2) and human stool (154) from Pantnagar, Nagla, Jawahar nagar, Lalkuan and Rudrapur were included in this study. Plasmid profile analysis of all Salmonella isolates revealed that majority of Salmonella isolates harboured 2 plasmids with size of 23.13 kb and above 23.13 kb. Four isolates of Salmonella harboured similar pattern of 5 plasmids of different sizes, in which each isolate harboured the plasmids of 2.027 kb,2.322 kb, 4.361 kb, 23.13 kb and more than 23.13 kb size. However, a different banding pattern, i.e. presence of single band was also exhibited by some Salmonella isolates in the present study. The plasmid profile analysis of Salmonella isolates indicated genetic similarities as well as genetic variations among some of these isolates.

Keywords: Plasmid profile, plasmid size, Salmonella serovars