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ABSTRACT

In this study, we describe the prevalence and characterisation of rotaviruses (RVs) isolated from cattle and buffalo calves of northern and central part of India. A total of 218 fecal samples collected from bovine calves (cow 157, buffalo 61) from the six states of India viz. Uttar Pradesh, Uttarakhand, Madhya Pradesh, Haryana, Himachal Pradesh, Jammu & Kashmir, were screened by polyacrylamide gel electrophoresis (RNA-PAGE). Wherein, 27.38% (43/157) cattle calves and 39.34% (24/61) buffalo calves were found RV positive, with an overall prevalence of 30.73% (67/218). However, none of the samples was detected positive from Himachal Pradesh and Jammu & Kashmir. In group specific VP6 gene based RT-PCR assay, 31.84% (50/157) cattle samples and 47.54% (29/61) buffalo samples were detected positive. Multiplex PCR based genotyping revealed predominance of G3 type (21.42%), followed by G6 (14.28%) and G10 (9.52%), while several mixed genotypes were also detected. Among the P types, P[11] was the predominant type (83.33%, 35/42), while 2 samples showed P[1]P[11] mixed P type (4.76%), P[1] in 4 (9.5%) samples, and one sample was non-typable. Overall, the findings of present study address some of the concern regarding the genotype dynamics and disease epidemiology of the circulating RVs in the bovine population from India.

Keywords: G and P genotyping, phylogeny, rotavirus, RT-PCR, VP6 gene