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Page No. : 19-23

ABSTRACT

Leptospirosis is an important zoonosis with broad host range. Dogs are one of the important maintenance hosts where the disease ranges from in-apparent to clinically overt. Globally, leptospirosis is misdiagnosed or under diagnosed in companion animals like dogs in developing countries such as India due to lack of rapid, affordable and user friendly diagnostic aids. Hence, it is high time to develop rapid, inexpensive and reliable pen-side diagnostic tests with high sensitivity, specificity and accuracy. In the present study, recombinant LigB protein based latex agglutination test has been evaluated for serodiagnosis of canine leptospirosis with results obtained within two minutes having proven sensitivity (83.47%), specificity (99.06%) and high degree of accuracy (94.56%). It pitches for its use as a rapid spot test following further validation in peripheral levels of animal health care system.

Keywords: Leptospirosis, recombinant LigB, dog