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Page No. : 29-35

ABSTRACT

In the present study, 100 pork samples including 50 each of ground pork and pig tonsils collected from the local market were examined by both PCR and culture method for presence of Yersinia enterocolitica. PCR assay based on the specific amplification of 16S rRNA gene and ail gene of Yersinia enterocolitica was carried out to amplify a chosen target DNA sequence. The detection limits of the PCR for Yersinia enterocolitica in experimentally inoculated ground pork was 104 CFU per g. without enrichment, 103 CFU per g. with enrichment for 12 h and 102 CFU per g. with enrichment for 24 h. On application of PCR for detection of Yersinia enterocolitica in pork, out of 100 samples, Yersinia enterocolitica was detected in 26 (26%). The overall percent positivity by PCR for detection of Yersinia enterocolitica from ground pork was 11 (22%) and pig tonsil 15 (30%) as compared to ground pork 8 (16%) and pig tonsil 10 (20%) by culture method, respectively.

Keywords: Y. enterocolitica, pork, isolation, PCR, tonsils.