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CITE
V.M. Vaidya, A.M. Paturkar, R.J. Zende, D.G. Dighe, R.N. Waghamare, D.V. Jundale, S.G. Panchal, S.L. Moon and S.S. Bhave .(2017). "Current Scenario of Trichinella Infection in Domestic Pigs, Rats and Humans of Maharashtra State". Journal of Veterinary Public Health, Vol. 15 Issue 1. Page No: 15-21
Current Scenario of Trichinella Infection in Domestic Pigs, Rats and Humans of Maharashtra State
Page No. : 15-21
ABSTRACT
The aim of the study was to know current scenario of Trichinella infection in domestic pigs, from different farms and slaughterhouses and rats living in and around pig farms and slaughterhouses as well as high risk humans in Maharashtra state, India. All together 1529 pigs and 162 rats (Rattus rattus) were screened by both Pepsin–HCL digestion and PCR assay where seven pig (0.46%) and ten rat (6.17%) diaphragms were found positive for Trichinella larvae. Sequence analysis revealed six pigs and ten rats harboured T. spiralis and one pig harboured T. pseudospiralis. Phylogenic analysis of sequencing data revealed that four isolates clustered with T. spiralis of previously published isolates confirming their species characteristics. Seroprevalence of 1.63% trichinellosis in pigs and 0.67% in humans was recorded by ELISA. This study indicated that pigs and rats play an important role in maintaining the cycle of Trichinella infection in animals.Keywords: Humans, Maharashtra, Pepsin-HCL digestion assay, pigs, rats, Trichinella spp.