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Page No. : 29-32

ABSTRACT

E. coli isolates of human and animal origin were studied for ESBL production, haemolytic and haemag-glutinating activity by phenotypic methods. E. coli were isolated from human urine samples (24%), and faecal samples of cattle (100%), pig (80%), and poultry (96%). The prevalence of ESBL positive E. coli was found to be 41.67% and 39.13% in human and animal, respectively. Among 24 human E. coli, haemolytic ability was seen in 6 (25%) isolates by plate method and 3 (12.5%) isolates by tube method. Out of 69 E. coli isolates of animal origin, 32 (46.38%) were found to be haemolytic by plate method and 34 (49.28%) by tube method. E. coli originating from human sources showed MRHA activity in 3 isolates (12.5%), while MSHA activity was absent. However, E. coli isolates of animal origin exhibited MRHA property in 25 isolates (36.23%) and MSHA property in 19 isolates (27.54%). The study shows that a significant number of E. coli isolates in animals as well as human beings are ESBL producers and are significantly pathogenic. Such organisms must be taken seriously while devising preventive and cure health strategies.

Keywords: E. coli., ESBL, haemolysis, MRHA, MSHA