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Page No. : 53-56

ABSTRACT

The genomic diversity and relationship among 63 Bacillus cereus isolates obtained from human stool, raw milk, meat and meat products were investigated by repetitive extragenic palindromic-PCR (rep-PCR) fingerprinting. A number of polymorphic bands were generated in the rep-PCR profiles, which resulted into development of a dendrogram with 9 major groups rep-type. In the present study, all the isolates belonging to human stool origin were clustered in rep-type Group-3, 5, 6, 7 and 9; isolates from meat samples were grouped together in rep-type Group-1, 2, 4, 5 and 8. Cluster analysis showed that all isolates from raw milk were clustered quite separately in rep-type Group-5 except two, which were present in rep-type Group-6 and 7. A significant variation in DNA polymorphism was noted on the basis of rep-PCR and it was also evident that the most of the isolates of same origin were clustered in same group. In the present study, rep-PCR is found as an efficient tool to identify the origin and epidemiological distribution of Bacillus cereus isolates.

Keywords: Bacillus cereus; human stool; meat; milk; repetitive extragenic palindromic-PCR