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N. Sahu, H.V. Murugkar, M. Kumar, A. Pathak, R. Sood, C. Tosh, S. Nagarajan and G. Kashyap.(2016). "Assessment of Viral Load in Meat from Turkeys Infected with Highly Pathogenic Avian Influenza (H5N1) Virus". Journal of Veterinary Public Health, Vol. 14 Issue 2. Page No: 61-64
Assessment of Viral Load in Meat from Turkeys Infected with Highly Pathogenic Avian Influenza (H5N1) Virus
Page No. : 61-64
ABSTRACT
A study was carried out to estimate the distribution of highly pathogenic avian influenza HPAI virus (H5N1) in meat from the infected turkeys. Three turkeys each were experimentally inoculated with two different virus isolates (A/duck/Tripura/103597/2008 and A/duck/India/02CA10/2011. The infected turkeys died within 72 h. To detect the virus load in different meat samples, viz., liver, thigh muscle and breast muscle, EID50 was estimated and Real-Time RT-PCR was carried out. The study revealed that all the meat samples contained a very high load of avian influenza virus and among the different types of samples; the viral load in the thigh muscle was considerably higher than that of liver and breast muscle. The presence of a high viral load in the meat samples might pose a significant occupational hazard for the persons handling the HPAI infected birds.Keywords: HSN1, influenza, real time, Turkey, viral load.