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CITE
A.A. Tekale, C.V. Savalia, D.P. Kshirsagar, M.N. Brahmbhatt and Y.A. Chatur .(2015). "Detection and Virulence Gene Characterization of Salmonella Isolates from Fish by Conventional and Molecular Methods". Journal of Veterinary Public Health, Vol. 13 Issue 1. Page No: 43-46
Detection and Virulence Gene Characterization of Salmonella Isolates from Fish by Conventional and Molecular Methods
Page No. : 43-46
ABSTRACT
Salmonella isolates (n=9) used in this study were isolated from 216 fish samples collected from retail market of Anand city, Gujarat, India. Salmonella serovars from fish were isolated and identified as per BAM USFDA method and serotyped at National Salmonella and Escherichia Centre (NSEC), Kasauli (Himachal Pradesh, India). Virulence factors of Salmonella serovars have been characterized with invA, spvR, spvC and stn genes using the polymerase chain reaction (PCR). All Salmonella serovars possessed 284 bp invA gene and 260 bp enterotoxin (stn) gene but lacks spvR or spvC gene. This showed that all Salmonella serovars isolated from fish were capable of producing gastroenteric illness to humans but were unable to produce systemic infection. The invA and stn genes might be the specific targets for Salmonella identification.Keywords: Enterotoxins, gastroenteris, PCR, Salmonella, virulence