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Page No. : 42-46

ABSTRACT

As foods of animal origin are major source of antimicrobial resistant E. coli responsible for various infections including urinary tract infections, a cross-sectional study was conducted from September 2020 to October 2021 to evaluate the occurrence of E. coli and its antibiotic resistance profi les in milk and milk products of Bareilly, U
ar Pradesh. A total of 90 milk and milk product samples (40 raw milk and 50 traditionally processed milk products) were collected and, processed for isolation and biochemical characterisation of E. coli. Kirby–Bauer disc diff usion technique was employed to determine antimicrobial susceptibility pa
ern using a panel of 21 antibiotics. Altogether, 18 (20%) isolates were confi rmed as E. coli by traditional culture method. Overall, the highest resistance was estimated against ampicillin (AM) 18/18 (100%), cefotaxime (CTX) 13/18 (72.22%), ceftazidime (CAZ), 13/18 (72.22%), tetracycline (TET) 16/18 (88.88%), cefi pime (FEP) 14/18 (77.77%) and pipercilline (PIP) 14/18 (77.77%), while majority of the E. coli isolates showed susceptibility to carbapenem antibiotics, amoxy-clav (AmC) and aztreonam (ATM). All the resistant isolates of E. coli reported as multidrug resistant. Minimum inhibitory concentration (MIC) values ranged from 0.001 to 256 μg/mL. As a result, the current study suggests the importance of antimicrobial susceptibility testing to treat the various animal diseases to avoid indiscriminate use of antibiotics.
Keywords: AMR, E. coli, milk, milk products