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CITE
Uttam Singh, D.K. Sinha, P. Chaudhury and V.P. Singh .(2015). "Comparative Study on Seroepidemiology of Caprine Leptospirosis by Microagglutination Test (MAT) and Recombinant LipL41 ELISA". Journal of Veterinary Public Health, Vol. 13 Issue 2. Page No: 75-78
Comparative Study on Seroepidemiology of Caprine Leptospirosis by Microagglutination Test (MAT) and Recombinant LipL41 ELISA
Page No. : 75-78
ABSTRACT
The recombinant leptospiral lipoprotein 41(rLipL41) was used as an antigen in enzyme-linked immunosorbent assay (ELISA) for serodiagnosis of caprine leptospirosis. Its results were compared with microscopic agglutination test (MAT). A total of 402 caprine sera samples were tested. The overall sero-prevalance of 10.44% and 15.67% were recorded by MAT and ELISA, respectively. The relative sensitivity, specificity of ELISA in comparison to MAT, was 100% and 94.17%, respectively. A high concordance percentage (94.77%) and substantial agreement by kappa statistics between MAT and ELISA results were observed. Thus rLipL41 ELISA could be used as a sero-diagnostic test for caprine leptospirosis.Key words: Caprine, ELISA, leptospirosis, MAT, recombinant lipoprotein