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CITE
A. Vijaya Kumar, N. Krishnaiah and L. Venkateswara Rao .(2010). "Comparative Efficacy of Cultural and PCR Methods for Detecting Salmonella in Livestock Products". Journal of Veterinary Public Health, Vol. 07 Issue 1. Page No: 59-62
Comparative Efficacy of Cultural and PCR Methods for Detecting Salmonella in Livestock Products
Page No. : 59-62
ABSTRACT
Livestock products were screened for Salmonella to compare the efficacy of cultural and PCR methods. A set of primers derived from inv A gene were employed to standardize the PCR assay for detection of Salmonella from livestock products, which gave specific amplification of a 389 bp fragment. Buffered peptone water as pre-enrichment broth followed by four different selective enrichment broths were used in this study. This two-step enrichment protocol gave good results both in cultural and PCR assay. Screening of 220 livestock products viz., milk, meat, chicken, egg swabs and fish revealed 48 and 61 samples positive for Salmonella by cultural and PCR assay, respectively.Keywords: Cultural method, invA gene, livestock products, PCR, Salmonella