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Page No. : 75-78

ABSTRACT

S. Typhimurium causes food-poisoning throughout the world. Oxidant bombardment is one of the important stresses encountered by S. Typhimurium inside the host. The oxidants tend to cause varying levels of oxidation in the protein repertoire of the cell causing the aggregation of proteins. The accumulation of aggregated proteins is toxic to cells. Caseinolytic proteases (Clp) clpA and clpB helps in degradation and disaggregation of aggregated proteins thus enhances cellular survival during stress. In current study, we deleted clpA and clpB genes from S. Typhimurium. The deletions were confirmed by PCR. Flanking primers to clpA gene amplified 296 bp product in ∆clpA strain and 2.4 kb in WT strain. Similarly, flanking primers to clpB gene amplified 566 bp in ∆clpB and 3 kb in WT strains respectively. These mutants can be utilised to decipher the roles of ClpA and ClpB genes in oxidative stress survival and virulence of Salmonella Typhimurim in future.

Keywords: clpA, clpB, mutants, oxidative stress, S. Typhimurium