-
Mail Address
vetviju@gmail.com
close
CITE
Irfan Ashraf, Mohd. Rashid* and Moien Javaid .(2017). "Molecular Characterization of Shiga Toxin Producing Escherichia coli (STEC) from Sheep, Goats and their Nomadic Handlers in the Jammu Region of Jammu and Kashmir". Journal of Veterinary Public Health, Vol. 15 Issue 2. Page No: 89-93
Molecular Characterization of Shiga Toxin Producing Escherichia coli (STEC) from Sheep, Goats and their Nomadic Handlers in the Jammu Region of Jammu and Kashmir
Page No. : 89-93
ABSTRACT
A total of 170 samples were collected from in and around Jammu region and processed for isolation of E. coli. A total of 65 E. coli isolates 27 of sheep, 26 of goats and 12 of humans were screened by multiplex polymerase chain reaction (mPCR) for the presence of stx1, stx2, eaeA and hlyA genes. Shiga toxin producing Escherichia coli (STEC) detected in 28 isolates, with prevalence rate of 55.5% and 50% in sheep and goats, respectively. All the isolates of nomadic handlers were negative for all genes and none of the sheep and goat isolates was found positive for eaeA gene.Keywords: Escherichia coli, goat, mPCR, nomadic handlers, primers, sheep, STEC.