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Page No. : 9-16

ABSTRACT

In an attempt to find a suitable bioassay model for the detection of Salmonella enterotoxin as well as cytotoxin, a total of 40 Salmonella isolates comprising of S. Typhimurium, S. Enteritidis, S. Saintpaul, S. Mabdakaa and S. Dublin, obtained from different sources in U.K. were screened using various in-vitro and one in-vivo assay models. None of the cell-free-culture-supernatants (CFCS) and cell-free-culture-filtrates (CFCF) prepared from these isolates was found to express enterotoxic activity in rabbit ligated ileal loop (RLIL). On exposure of cell cultures, viz., Chinese hamster ovary (CHO), Y-1 mouse adrenal, African green monkey kidney (VERO) and embryonated bovine lung (EBL) cells with CFCS and CFCF of 40 strains of Salmonella, 12 CFCS and CFCF showed a cytotoxic response on CHO and EBL cells. Only three preparations could produce toxic effect on VERO cells. No such response was, however, observed in Y-1 mouse adrenal cells. The cytotoxic preparations were found to either kill or lyse the CHO and EBL cells, whereas the affected VERO cells appeared to be round and shriveled.

Keywords: Salmonella, Cytotoxin, Enterotoxin, Cell Culture, CHO cells, EBL cells, Y-1 mouse adrenal cells, VERO cells.