Archives

Page No. : 19-23

ABSTRACT

Acinetobacter baumannii has emerged as a major nosocomial pathogen that poses a serious hazard to public health globally. Despite the availability of many selective media for the isolation and identification of A. Baumannii from clinical samples, isolation from animal faeces remains difficult and time-consuming. The performance of four bacteriological media for the isolation of A. Baumannii from cattle faeces samples was tested in this study. We collected 62 fresh rectal swabs from healthy cattle and used HiCrome Acinetobacter Agar base. Leeds Acinetobacter Medium (LAM), 5% sheep blood agar (SBA), and MacConkey agar supplemented with 1 mg/L cefotaxime to isolate A. baumannii. From cattle faecal samples, LAM and SBA detected 5 of 5 (100%) A. baumannii isolates, followed by HiCrome Acinetobacter Agar Base detected 4 of 5 (80%) A. baumannii isolates and MacConkey agar supplemented with 1 mg/L cefotaxime recovered 2 of 5 (40%) isolates. Various biochemical assays and polymerase chain reaction targeting both genus and species specific genes were used to confirm all of the isolates. Overall, LAM has been found to be an excellent growing medium for selectively isolating A. Baumannii from animal faeces samples. Furthermore, LAM is more cost-effective, less time-consuming, and simple to use, especially when screening a large number of samples.

Keywords: Acinetobacter baumannii; animals; isolation; media; LAM