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Page No. : 40-47

ABSTRACT

The present study was conducted to determine occurrence of Escherichia coli in faecal samples of bovine calves reared under different farming systems in Bareilly city and their antimicrobial drug resistance pa erns with special emphasis on extended-spectrum beta-lactamase (ESBL) producing multidrug resistant (MDR) E. coli. A total of 90 faecal samples from diarrheic and non-diarrheic calves (up to three months of age) were collected from different organized and unorganized dairy farms located in Bareilly city. The samples were processed for isolation and characterization of E. coli. Antibiotic sensitivity of the isolates was performed using disk diffusion method against 17 antibiotics followed by determination of ESBL producing MDR E. coli. A total of 344 isolates of E. coli were isolated and the antibiogram of these isolates revealed that most of them showed resistance to ampicillin (65.57%) followed by cefotaxime (39.52%), nalidixic acid (26.95%), tetracycline (23.65%), amoxicillin (19.76%) and ciprofloxacin (17.37%). Chloramphenicol was the most effective drug which inhibited 99.1% isolates. Approximately 15.27 % of isolates were MDR E. coli which were resistant to three or more antibiotics. Surprisingly, 220 (65.87%) isolates were found to be ESBL producing E. coli. At present, the drug resistance considered as a serious global concern MDR strains and ESBL producing E. coli from bovine calves suggests that there is a need to continuous vigilance over emergence of drug resistance in E. coli as it is an emerging pathogen of zoonotic significance in current era of antibiotic resistance.

Keywords: Antibiogram, E. coli, ESBL, MDR