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Page No. : 48-54

ABSTRACT

Viral acute-gastroenteritis is one of the most common morbidity and mortality in infants/ young children and many animal species. Being double-stranded RNA viruses, rotavirus is the most common and leading cause of acute watery dehydrating diarrhoea noticed often in the valley of Kashmir. The present study was carried out to screen the diarrhoeic samples from calves (< 1 yr) (n=100) for the presence of Group A rotaviruses with its molecular typing of strain based on structural genes (VP7 and VP4). Eleven samples were found positive for the presence of the virus using Latex Agglutination Test (LAT). Genotypic characterization (VP7 gene, G genotype) of the rotaviruses revealed predominance of G10 (44.4%) followed by G6 (22.2%) and G3 (11.15%). Characterization of VP4 gene (P genotype) revealed dominance of P[11] (55.5%) followed by P[5] (22.2%). The genotypic combination from the positive samples of calves (bovine) presented as G10P [11] and G6P [11] to be 22.2% followed by G10P [5] and G3P[11] as 11.1%.

Key words: Bovine, children, LAT, PCR, rotavirus