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B.G. Mane, V.K. Tanwar, P.S. Girish and V.P. Dixit
.(2006). "Identification of Species Origin of Meat by RAPD-PCR Technique ". Journal of Veterinary Public Health, Vol. 04 Issue 2. Page No: 87-90
Identification of Species Origin of Meat by RAPD-PCR Technique
Page No. : 87-90
ABSTRACT
RAPD-PCR was applied for the identification of different meat species (buffalo, cattle, sheep, goat, pig and chicken) in fresh and frozen meat samples. Six random primers were used for RAPD-PCR amplification of DNA extracted from fresh and frozen meat. The primer RPN2 showed polymorphism with unique and species-specific banding pattern in cattle (312 bp and 615 bp), pig (724 bp), sheep (835 bp) and chicken (480 bp), which was sufficient enough to differentiate the meat species. The other primers were monomorphic showing similar amplification pattern and thus were unable to differentiate species origin of meat. The result showed that the RAPD-PCR with primer RPN2 could be a useful technique for meat species identification.Keywords: Meat species, random primer, RAPD-PCR.