Archives

Page No. : 1-7

ABSTRACT

A High-Performance Liquid Chromatography (HPLC) method was standardized for the detection of chloramphenicol residues from meat, liver and kidney of common food animals using Symmetry C18 column and acetonitrile: water (20:80) as mobile phase. A variable wavelength UV detector at 278 nm was used for the detection and quantification of chloramphenicol residue. The average retention time (Rt) of standard chloramphenicol observed was 7.37±0.09 minutes. Level of detection (LOD) of chloramphenicol in spiked meat, liver and kidney samples was found to be 25 ng/g. Overall recovery of the drug for all concentrations was 62.61%, 75.99% and 63.00% for meat, liver and kidney tissue, respectively. Thus, the method can be effectively used for preliminary screening of field samples and detection of chloramphenicol concentrations above 25 ng/g.

Keywords: Chloramphenicol, food animals, HPLC, kidney, liver, meat