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Page No. : 101-105

ABSTRACT

The present Investigation was undertaken to study the antilisterial activity of Lactobacillus acidophilus 291 in buffalo meat. Trials were conducted using three different concentrations of L. acidophilus 291 viz. 105, 106 and 107 cells/ml. Buffalo meat samples were decontaminated with hot water for 10 min at 100°C and were spiked with 3.0 log10 cu/g of Listeria monocytogenes. Treated meat samples were inoculated with L. acidophilus 291 at above concentrations and were stored at 4±1°C for 21 days. The growth of L. monocytogenes in the treated samples of group 1, 2 and 3 was significantly (P<0.05) inhibited when compared to the control group. The estimate of pH in L. acidophilus 291 and L. monocytogenes inoculated meat samples was 5.23, 4.90, 4.68 in groups 1, 2 and 3, respectively, which remained significantly (P<0.05) lower than the control group. The degree of inhibition of L. monocytogenes increased with increasing concentration of L. acidophilus 291. These studies demonstrate that the L. acidophilus 291 is effective in controlling the growth of L. monocytogenes in buffalo meat.

Keywords: Buffalo meat, Lactobacillus acidophilus 291, Listeria monocytogenes, growth inhibition.