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CITE
H. Rahman, R.H. Begum, H. V. Murugkar, H. Rahman, A. Kumar, B.R Shome and R. Shome.(2008). "Occurrence and Distribution of Virulence Factors among Clinical Isolates of Non-O157 E. coli". Journal of Veterinary Public Health, Vol. 06 Issue 1. Page No: 9-13
Occurrence and Distribution of Virulence Factors among Clinical Isolates of Non-O157 E. coli
Page No. : 9-13
ABSTRACT
One hundred thirty one non-O157 E. coli isolated from clinical cases in man (15), piglets (78) and calves (38) were tested for the presence of virulence genes viz., stx1, stx2, est1, elt2, and hlyA by PCR using their specific primers. The isolates belonged to 51 different serotypes and 20.61% of them were found refractory for serotyping. Serotype O117 was encountered most frequently (4.58%) and no clustering of any serotype was seen. PCR results showed that est gene was the most prevalent (22.9%) virulence gene among the isolates. Isolates from piglets contributing the highest percentage among the positives. The hlyA gene could be detected in 4 isolates, all from calf (O103, O168, O172). One of the positive isolate was untypable. The stx1 gene could be found in only one isolate. The isolate was from piglet (O172) and was also positive for est gene. The stx1 gene was detected in only one isolate (O5).Keywords: Calves, Escherichia coli, human, pathogenic, PCR, piglets, serotype, virulence genes (est1, elt2, hlyA, stx1, stx2)