-
Mail Address
vetviju@gmail.com
close
CITE
R.S. Barge, V.S. Waskar, A.T. Sherikar, A.M. Paturkar and R.J. Zende
.(2008). "Surveillance of Bovine Muscle and Organ Samples for the Presence of Residues of Gentamicin Sulfate by HPLC". Journal of Veterinary Public Health, Vol. 06 Issue 2. Page No: 57-61
Surveillance of Bovine Muscle and Organ Samples for the Presence of Residues of Gentamicin Sulfate by HPLC
Page No. : 57-61
ABSTRACT
Cattle (male only) and buffalo muscle/organ samples were subjected to solid phase extraction, clean-up procedure and derivatization prior to chromatographic analysis. High performance liquid chromatographic technique employing acetonitrile:water as mobile phase, C18 column and fluorescence detector was standardised for detection and quantitation of gentamicin residues. Gentamicin was eluted in 4 components viz. C1, C1a C2a, and C2, within 15 min with lowest level of detection 50 ng/g. Recoveries ranged between 41.89-48.65% in muscle, 45.23-61.23% in liver and 39.82-44.61% in kidney. Violative amounts of gentamicin residues in muscle and organ samples of cattle and buffalo was found in 4.44% of samples.Keywords: Gentamicin, HPLC, kidney, liver, muscle