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CITE
A. Vijaya Kumar, R. Annie Supriya, N. Krishnaiah and I. Venkateswara Rao.(2009). "Detection of Salmonella Typhimurium in Artificially Inoculated Chicken Meat by PCR Technique". Journal of Veterinary Public Health, Vol. 07 Issue 2. Page No: 125–127
Detection of Salmonella Typhimurium in Artificially Inoculated Chicken Meat by PCR Technique
Page No. : 125–127
ABSTRACT
A set of primers derived from fliC gene was employed to standardize the PCR assay for detection of Salmonella Typhimurium in artificially inoculated chicken meat. In the present study, the minimum detection level of Salmonella Typhimurium was found to be 3.7 cfu/ml or 0.01 cfu per PCR reaction and enrichment in tetrathionate broth was observed to be a suitable broth to detect very low levels of Salmonella.Keywords: fliC gene, PCR technique, Salmonella Typhimurium, sensitivity, specificity