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CITE
S. Rawat, K.N. Bhilegaonkar, R.K. Agarwal, Ashok Kumar, Z.B. Dubal, M. Singh, R. Biswas and Lokesh K.M..(2012). "Prevalence of group A rotavirus in bovine calves of North India". Journal of Veterinary Public Health, Vol. 10 Issue 1. Page No: 7-10
Prevalence of group A rotavirus in bovine calves of North India
Page No. : 7-10
ABSTRACT
The present study was undertaken with the objective to determine the role of rotavirus in causing diarrhoea in bovine calves (< 3 months of age). A total of 105 samples, from three states of India, namely Uttar Pradesh, Uttarakhand and Haryana were screened for the presence of viral nucleic acid, using polyacrylamide gel electrophoresis (PAGE) and reverse transcription-polymerase chain reaction (RT-PCR). The study revealed that 14 (13.3%) bovine calves were positive for rotavirus, with 13 (12.4%) positive by PAGE and 12 (11.4%) positive by RT-PCR. Two and one samples were found to be exclusively positive by PAGE and RT-PCR, respectively.Keywords: Bovine rotavirus, PAGE, prevalence, RT-PCR