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Page No. : 65-71

ABSTRACT

Campylobacter jejuni is one of the leading causes of bacterial foodborne infections in the world today. Several case control studies have suggested the handling and consumption of contaminated poultry meat as one of the important routes of transmission to humans. The present study was conducted to investigate the genetic diversity among 29 Campylobacter jejuni isolates of chicken origin using two PCR-based genotyping techniques. The fla-typing method using restriction enzyme HinfI revealed a total of 18 fla-types, with all the isolates under study found typeable. Randomly amplified polymorphic assay using a universal primer (HLWL85) clustered the isolates into 24 types, with one isolate found untypable. The isolates under study revealed significant molecular heterogeneity and fla-typing proved to be a useful, relatively simple and reliable technique for subtyping C. jejuni among the two methods.

Keywords: Genotyping, fla-typing, molecular heterogeneity, RAPD