Archives

Page No. : 40-45

ABSTRACT

Species-specific polymerase chain reaction (PCR) assays were developed for the identification and differentiation of cattle meat from common meat species. Species-specific primer pairs were designed by targeting mitochondrial D-loop and cytochrome b gene of ca fi cation of amplicon sizes of 305 bp and 647 bp. The optimized PCR assays were subsequently validated for their specificity and sensitivity and found to be devoid of any cross-reactivity with buffalo, sheep, goat, and pig. The analytical sensitivity of developed assay was determined by making 10 folds serial dilution of DNA sample of cattle meat and estimated as 10 ng of absolute DNA. The Developed species-specific PCR assays were also useful in the detection of cattle meat in meat admixture samples. The specificity of cattle specific assays provide valuable tools for the identification of cattle meat and check against fraudulent substitution, adulteration, and mislabeling.

Keywords: Adulteration detection, cytochrome B gene, meat speciation, mitochondrial D-loop gene, PCR