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Page No. : 63-67

ABSTRACT

Yersinia enterocolitica is an important food-borne enteropathogen of human and animals having worldwide significance. In the present study twenty-two Y. enterocolitica isolates from pig and human samples were confirmed using species specific PCR by targeting 16S rRNA gene with an expected amplicon of 330 bp. Further, the genetic diversity of these isolates was assessed by using ERIC-PCR and REP-PCR. Both the genotyping methods showed diversity among Y. enterocolitica isolates. ERIC-PCR revealed the presence of 12 different genotypes with a Discriminatory power (D) of 0.9567 and Shanon Weaver index (H’) of 2.46 among isolates. REP-PCR revealed 14 genotypes with a Discriminatory Power of 0.9667 and Shanon Weaver index of 2.58 indicating REP-PCR is found to be more discriminatory for genotyping of Y. enterocolitica than the ERIC-PCR. Based on the data analysis obtained from these methods, the isolates from the same source are grouped into different cluster (genotype) or isolates from different sources are grouped into same cluster. Further, grouping of human isolates along with the pig tongue isolates into same cluster is indicative of circulation of same genotype among human and animals. However, these typing methods are fast and reliable for the study of heterogeneity among the Yersinia isolates.

Keywords: Discriminatory Power, ERIC-PCR, pork, REP-PCR, Shanon Weaver index, Yersinia enterocolitica