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Page No. : 68-71

ABSTRACT

The present investigation was carried out to study antibiotic residue in bovine milk in and around Mhow, district Indore (Madhya Pradesh). In this study, a total of 180 bovine milk samples were collected from different locations viz. Indore, Mhow, Sanwer and Depalpur areas nearby Mhow (MP) using a systematic random sampling technique. Milk samples were screened for antibiotic residues by microbial inhibition assay using agar well diffusion method. Out of total, 94.44% milk samples were found to be negative for antibiotic residues i.e. no visible transparent zone of inhibition around the wells in the Muller Hinton agar surface, while 5.55% milk samples were found to be positive for the presence of antibiotic residues with mean zone of inhibition size 10.00 mm. In the Mhow and Depalpur area, 3.33% of milk samples were found to be positive followed by Indore and Sanwer area where 6.66% and 10.00% of milk samples were found to be positive for antibiotic residues, respectively. Area-wise status of antibiotic residue in milk samples in and around Mhow showed that 6.66% and 3.33% of milk samples were found to be positive in the rural and urban areas, respectively. Sector-wise status of antibiotic residue in milk samples in and around Mhow area showed that 4.16% and 5.76% of milk samples were found to be positive in an organized sector and unorganized sector, respectively. In the current study, all the positive milk samples were found to be non-compliant with ideal food safety norms which denote a condition of being free from physical contaminants, chemical residues and pathogenic microbes. This study suggests that antibiotic residues present in milk samples may result in different health complications.

Keywords: Antibiotic residue, bovine milk, microbial inhibition